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Kasvibiotekniikka

Käytä uusia, luonnollisesti tuotettuja kasviperäisiä bioaktiivisia yhdisteitä ja ainesosia!

​Kehitämme kasveja, kasvisoluviljelmiä ja leviä biomassan, ainesosien sekä arvokkaiden kemikaalien ja proteiinien tuottamiseen tarpeittenne mukaisesti. Lähestymistapamme on luontoa säästävä, kestävä, turvallinen sekä hallittu. Tuloksena on taloudellisia ja ympäristöystävällisä tuotteita. Tehokas uudistuvien luonnonvarojen käyttö pitää kilpailukykyänne yllä ja mahdollistaa immateriaalioikeuksien luomisen. Ota siis askel eteenpäin ja käytä uusia, luonnollisesti tuotettuja kasviperäisiä bioaktiivisia yhdisteitä ja ainesosia. 

”Yhteistyö VTT:n kanssa on ollut erittäin antoisaa” toteaa Tiina Isohanni, Lumenen innovaatio- ja tuotekehitysjohtaja. ”Biotekniikan avulla olemme voineet huomattavasti laajentaa raaka-ainevalikoimaamme. Suunnittelemme jo yhteistyön jatkumista uusissa projekteissa”.

Ainutlaatuinen osaamisemme

Ainutlaatuinen osaamisemme kattaa koko ketjun innovaatiosta lopulliseen tuotteeseen. Käytössämme ovat teollisuusmittakaavan kasvisoluviljelylaitteistot. Tuotteiden kehittämisessä voidaan hyödyntää metabolian muokkausta soveltaen synteettisen biologian mahdollisuuksia sekä laaja-alaista kemiallista karakterisointia uusimman teknologian mukaisilla laitteillamme. Lisäksi hallitsemme biotransformaation ja kasvisolujen kylmäsäilönnän sekä erilaiset bioaktiivuustestaukset.  Arvokkaat kasvisolulinjamme löytyvät VTT:n kantakokoelmasta (VTT Culture Collection). Tärkeimmät tutkimuksemme fokusalueet ovat kasvi- ja meriperäiset luonnonaineet, terapeuttiset proteiinit sekä pohjoisen luonnon monimuotoisuuden hyödyntäminen esimerkiksi marjatutkimuksen muodossa.

Tilat ja laitteet

Tärkeimmät resurssimme ovat kasvi- ja kasvisoluviljelmäkokoelmamme, aseptiset tilat, modulaariset molekyylibiologian menetelmät, bioreaktorit (teräksiset, lasiset, kertakäyttöiset), kasvatushuoneet, kasvihuone- ja viljelykaapit, pakkas- ja nestetyppisäilytystilat ja analyyttinen infrastruktuuri (mm. UPLC-MS, GC-MS, GCxGC-ToF). Olemme verkottuneita muun muassa EPSOn (European Plant Science Organisation), ISPMFn (International Society for Plant Molecular Farming), EU COST-Actionien sekä kasvitieteen tohtoriohjelman kanssa. Projektimme liittyvät useisiin eri teollisuutta edustaviin kokonaisuuksiin kuten kemia, farmasia, kosmetiikka-, ravitsemus-, juoma- sekä rehuteollisuus sekä mallastus- ja panimo- sekä aromi- ja hajusteteollisuus. 

Tieteelliset julkaisut

2016 

 

Fermentation and dry fractionation increase bioactivity of cloudberry (Rubus chamaemorus)
Puupponen-Pimiä, R., Nohynek, L., Juvonen, R., Kössö, T., Truchado, P., Westerlund-Wikström, B., Leppänen, T., Moilanen, E. & Oksman-Caldentey, K.-M.
Food Chemistry 2016 Apr 15;197(PtA):950-958. doi: 10.1016/j.foodchem.2015.11.061
http://www.ncbi.nlm.nih.gov/pubmed/26617039

 

Continuous flow separation of hydrophobin fusion proteins from plant cell culture extract.
Reuter, L., Conley, A. & Joensuu, J.J.
Methods in Molecular Biology (Clifton, N.J.) 2016;1385:189-197. doi: 10.1007/978-1-4939-3289-4_14
http://www.ncbi.nlm.nih.gov/pubmed/26614291

 

The N-glycan on Asn54 affects the atypical N-glycan composition of plant-produced interleukin-22, but does not influence its activity
Wilbers, R.,  Westerhof, L., Reuter, L., Castilho, A., van Raaij, D., Nguyen, D., Lozano-Torres, J., Smant, G., Hokke, C., Bakker, J. & Schots, A.
Plant Biotechnol Journal 2016 Feb; 14(2):670-81. doi: 10.1111/pbi.12414
http://www.ncbi.nlm.nih.gov/pubmed/26059044

 

2015

Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents
Natunen K, Seppälä J, Schwenk D, Rischer H, Spilling K, Tamminen T.
Journal of Applied Phycology 2015;27(3):1161-1168. doi:10.1007/s10811-014-0404-5
http://www.ncbi.nlm.nih.gov/pubmed/25983393

 

Analysis of beers from an 1840s' shipwreck
Londesborough, J., Dresel, M., Gibson, B., Holopainen, U., Mikkelson, A., Seppänen-Laakso, T., Viljanen, K., Virtanen, H., Wilpola, A., Kivilompolo, M., Hoffmann, T. & Wilhelmson, A.
 Journal of Agricultaral Food Chemistry 2015 Mar 11;63(9): 2525-2536. doi: 10.1021/jf5052943 http://www.ncbi.nlm.nih.gov/pubmed/25664918

 

Optimization of invasion-specific effects of betulin derivatives on prostate cancer cells through lead development
Härmä, V.V., Havikko, R., Virtanen, J. Ahonen, I., Alakurtti, S., Schukov, H.-P., Purev, E., Rischer, H., Yli-Kauhaluoma, J, Moreira, V.M., Nees, M. & Oksman-Caldentey, K.-M.
PloS One 2015 May 12;10(5):e0126111. doi: 10.1371/journal.pone.0126111
http://www.ncbi.nlm.nih.gov/pubmed/25965345

 

Determination of terpenoid indole alkaloids in hairy roots of Rhazya stricta (Apocynaceae) by gas chromatography- mass spectroscopy
Akhgari, A., Laakso, I., Seppänen-Laakso, T., Yrjönen, T., Vuorela, H., Oksman-Caldentey, K.-M. & Rischer, H. (2015):
Phytochemical Analysis 2015 Sep-Oct; 26(5): 331-338. doi: 10.1002/pca.2567
http://www.ncbi.nlm.nih.gov/pubmed/26095837

 

Physicochemical characterization of sahti, an "ancient" beer style indigenous in Finland
Ekberg, J., Gibson, B., Joensuu, J., Krogerus, K., Magalhaes, F., Mikkelson, A., Seppänen-Laakso, T. & Wilpola, A.
Journal of The Institute of Brewing 11 Sep 2015 121: 464-473. doi: 10.1002/jib.246
http://onlinelibrary.wiley.com/doi/10.1002/jib.246/abstract

 

Bioactivity and chemical characterization of gonads of green sea urchin Strogylocentrotus droebachiensis from Barents Sea
Pozharitskaya, O.N., Shikov, A.N., Laakso, I., Seppänen-Laakso, T., Makarenko, I.E., Faustova, N.M., Makarova, M.N. & Makarov, V.G.
Journal of Functional Foods 17, August 2015: 227-234. doi:10.1016/j.jff.2015.05.030
http://www.sciencedirect.com/science/article/pii/S1756464615002686

 

The bHLH factor BIS1 regulates the seco-iridoid pathway branch of monoterpenoid indole alkaloid biosynthesis in Catharanthus roseus.
Van Moerkercke, A., Steensma, P., Schweizer, F., Pollier, J., Gariboldi, I., Payne, R. Vanden Bosschem, R., Miettinen, K., Espoz, J., Candra Purnama, P., Kellner, F., Seppänen-Laakso, T., O'Connor, S., Rischer, H., Memelink, J. & Goossens, A.
Proceedings of the National Academy of Sciences USA 2015 Jun 30;112(26):8130-8135. doi: 10.1073/pnas.1504951112
http://www.ncbi.nlm.nih.gov/pubmed/26080427

 

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production
Akhgari, A., Yrjönen, T., Laakso, I., Vuorela, H., Oksman-Caldentey, K.-M. & Rischer, H.
Plant Cell Reports 2015 Nov;34(11):1939-1952. doi: 10.1007/s00299-015-1841-6
http://www.ncbi.nlm.nih.gov/pubmed/26245531

 

Polyketide synthases from poison hemlock (Conium maculatum L.)
Hotti, H., Seppänen-Laakso, T., Arvas, M., Teeri, T. & Rischer, H.
The FEBS Journal 2015 Nov;282(21):4141-4156. doi: 10.1111/febs.13410
http://www.ncbi.nlm.nih.gov/pubmed/26260860

 

Standards for plant Synthetic Biology:  A common syntax for exchange of DNAparts
Patron, N.J., Orzaez, D., Marillonnet, S., Warzecha, H., Matthewman, C., Youles, M., Raitskin O., Leveau, A., Farré, G., Rogers, C., Smith, A., Hibberd, J., Webb, A.A.R., Locke, J., Schornack, S., Ajioka, J., Baulcombe D.C., Zipfel, C.,  Kamoun, S., Jones, J.D.G., Kuhn, H., Robatzek, S., Van Esse, H.P. ,Oldroyd, G., Martin, C., Field, R., O'Connor, S., Fox, S., Wulff, B., Miller, B., Breakspear, A., Radhakrishnan, G., Delaux, P.-M., Loque, D., Granell A., Tissier, A., Shih, P., Brutnell, T.P., Quick, P., Rischer, H., Fraser, P.D., Aharoni, A., Raines, C., South, P., Ané, J.-M., Hamberger, B.R., Langdale, J., Stougaard, J., Bouwmeester, H., Udvardi, M., Murray, J.A.H., Osbourn A. & J. Haseloff, J.  
The New Phytologist 2015 Oct;208(1): 13-19. doi: 10.1111/nph.13532
http://www.ncbi.nlm.nih.gov/pubmed/26171760

 

Elicitation of furanocoumarins in poison hemlock (Conium maculatum L.) cell culture
Meier, P., Hotti, H. & Rischer H.
Plant Cell, Tissue and Organ Culture (PCTOC) 2015 Dec;123(3):443-453. doi:10. 1007/ s11240-015-0847-7
http://link.springer.com/article/10.1007%2Fs11240-015-0847-7

 

Bioconversion to raspberry ketone is achieved by several non-related plant cell cultures
Häkkinen, S.T., Seppänen-Laakso, T., Oksman-Caldentey, K.-M. & Rischer, H.
Frontiers Plant Sci. 2015 Nov 24;6:1035, doi: 10.3389/fpls.2015.01035
http://www.ncbi.nlm.nih.gov/pubmed/26635853

 

Increased dihydroceramide/ceramide ratio mediated by defective expression of degs1 impairs adipocyte differentiation and function
Barbarroja, N., Rodrigues-Cuenca, S., Nygren, H., Camargo, A., Pirraco, A., Relat, J., Cuadrado, I., Pellegrinelli, V., Medina-Gomez, G., Lopez-Pedrera, C., Tinahones, F.J., Symons, J.D., Summers, S.A., Oresic, M. & Vidal-Puig, A.
Diabetes 2015 Apr;64(4):1180-1192, doi: 10.2337/db14-0359
http://www.ncbi.nlm.nih.gov/pubmed/25352638

 

Harvinaisten ja vaikeasti syntetisoitavien lääkeyhdisteiden tuottaminen kasvibiotekniikan keinoin
Häkkinen, S.T., Oksman-Caldentey, K.-M., Ritala, A.
Dosis 2015 vol 31: 162-169

 

Analysis of indole alkaloids from Rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry
Akhgari, A., Laakso , I., Seppänen-Laakso, T., Yrjönen, T., Vuorela, H., Oksman-Caldentey, K.-M. & H. Rischer
Molecules 2015 Dec 17;20(12):22621-22634, doi: 10.3390/molecules201219873
http://www.ncbi.nlm.nih.gov/pubmed/26694342

 

Biotechnology of the medicinal plant, Rhazya stricta
Akhgari, A
Dosis 2015 Vol 31: 207-214

 

Serum lipid and serum metabolite components in relation to anthropometric parameters in EPIC-Potsdam participants.
Foerster, J., Hyötyläinen, T., Oresic, M., Nygren, H., Boeing, H.
Metabolism 2015 Oct;64(10):1348-1358, doi: 10.1016/j.metabol.2015.07.004
http://www.ncbi.nlm.nih.gov/pubmed/26271139


Disposable bioreactors for the cultivation of plant cell cultures.
Lehmann N, Dittler I, Lämsä M, Ritala A, Rischer H, Eibl D, Oksman-Caldentey K-M, Eibl R.
In: Production of biomass and bioactive compounds using bioreactor technology.
Paek KY, Murthy HN, Zhong JJ (eds.)
Springer Science + Business Media B.V., Dordrecht (in press)

 

2014


Nile Red staining of phytoplankton neutral lipids: species-specific fluorescence kinetics in various solvents
Natunen K, Seppälä J, Schwenk D, Rischer H, Spilling K, Tamminen T.
Journal of Applied Phycology. (2014) doi:10.1007/s10811-014-0404-5
http://link.springer.com/article/10.1007%2Fs10811-014-0404-5


The seco-iridoid pathway from Catharanthus roseus.
Miettinen K, Dong L, Navrot N, Schneider T, Burlat V, Pollier J, Woittiez L, van der Krol S, Lugan R, Ilc T, Verpoorte R, Oksman-Caldentey KM, Martinoia E, Bouwmeester H, Goossens A, Memelink J, Werck-Reichhart D.
Nat Commun. 2014 Apr 7;5:3606. doi: 10.1038/ncomms4606.
http://www.ncbi.nlm.nih.gov/pubmed/24710322


Ultrahigh-performance liquid chromatography-mass spectrometry in Lipidomics.
Nygren H, Pöhö, P; Seppänen-Laakso T, Lahtinen U, Oresic M, Hyötyläinen T.
LCGC North America. Advanstar Communications Inc. (2010) vol. 32:4, pp. 286-293.
 
Cloudberry (Rubus chamaemorus) cell culture with bioactive substances: Establishment and mass propagation for industrial use.
Nohynek L, Bailey M, Tähtiharju J, Seppänen-Laakso T, Rischer H, Oksman-Caldentey K-M, Puupponen-Pimiä R.
Engineering in Life Sciences. (2014) vol. 14:6, pp. 667-675. doi:10.1002/elsc.201400069
http://onlinelibrary.wiley.com/doi/10.1002/elsc.201400069/pdf


Molecular pharming in plants and plant cell cultures: a great future ahead?
Ritala A, Häkkinen ST, Schillberg S.
Pharmaceutical bioprocessing. (2014) vol. 2:3, pp. 223-226. doi:10.4155/PBP.14.21
http://www.future-science.com/doi/pdf/10.4155/pbp.14.21


Selection and validation of reference genes for transcript normalization in gene expression studies in Catharanthus roseus.
Pollier J, Vanden Bossche R, Rischer H, Goossens A.
Plant Physiol Biochem. 2014 Oct;83:20-5. doi: 10.1016/j.plaphy.2014.07.004. Epub 2014 Jul 10.
http://www.ncbi.nlm.nih.gov/pubmed/25058454


Quorum sensing signalling and biofilm formation of brewery-derived bacteria, and inhibition of signalling by natural compounds.
Priha O, Virkajärvi V, Juvonen R, Puupponen-Pimiä R, Nohynek L, Alakurtti S, Pirttimaa M, Storgårds E.
Curr Microbiol. 2014 Nov;69(5):617-27. doi: 10.1007/s00284-014-0627-3. Epub 2014 Jun 19.
http://www.ncbi.nlm.nih.gov/pubmed/24944110


Algae-bacteria association inferred by 16S rDNA similarity in established microalgae cultures.
Schwenk D, Nohynek L, Rischer H.
Microbiologyopen. 2014 Jun;3(3):356-68. doi: 10.1002/mbo3.175. Epub 2014 May 5.
http://www.ncbi.nlm.nih.gov/pubmed/24799387


Evaluation of tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) hairy roots for the production of geraniol, the first committed step in terpenoid indole alkaloid pathway.
Ritala A, Dong L, Imseng N, Seppänen-Laakso T, Vasilev N, van der Krol S, Rischer H, Maaheimo H, Virkki A, Brändli J, Schillberg S, Eibl R, Bouwmeester H, Oksman-Caldentey KM.
J Biotechnol. 2014 Apr 20;176:20-8. doi: 10.1016/j.jbiotec.2014.01.031. Epub 2014 Feb 14.
http://www.ncbi.nlm.nih.gov/pubmed/24530945


Relation of sensory perception with chemical composition of bioprocessed lingonberry.
Viljanen K, Heiniö RL, Juvonen R, Kössö T, Puupponen-Pimiä R.
Food Chem. 2014 Aug 15;157:148-56. doi: 10.1016/j.foodchem.2014.02.030. Epub 2014 Feb 14.
http://www.ncbi.nlm.nih.gov/pubmed/24679764


Comparison of plant-based expression platforms for the heterologous production of geraniol.
Vasilev N, Schmitz C, Dong L, Ritala A, Imseng N, Häkkinen S, van der Krol S, Eibl R, Oksman-Caldentey K-M, Bouwmeester H, Fischer R, Schillberg S.
Plant Cell, Tissue and Organ Culture. (2014) vol. 117:3, pp. 373-380. doi:10.1007/s11240-014-0446-z
http://link.springer.com/article/10.1007/s11240-014-0446-z


Recombinant barley-produced antibody for detection and immunoprecipitation of the major bovine milk allergen, β-lactoglobulin.
Ritala A, Leelavathi S, Oksman-Caldentey KM, Reddy VS, Laukkanen ML.
Transgenic Res. 2014 Jun;23(3):477-87. doi: 10.1007/s11248-014-9783-2. Epub 2014 Feb 5.
http://www.ncbi.nlm.nih.gov/pubmed/24497085


Phenolic metabolites as compliance biomarker for polyphenol intake in a randomized controlled human intervention.
Vetrani C, Rivellese AA, Annuzzi G, Mattila I, Meudec E, Hyötyläinen T, Oresic M, Aura A-M.
Food Research International. (2014) vol. 63 Part B, pp. 233 - 238. doi:10.1016/j.foodres.2014.01.018
http://www.sciencedirect.com/science/article/pii/S0963996914000246


Metabolic flux phenotype of tobacco hairy roots engineered for increased geraniol production.
Masakapalli SK, Ritala A, Dong L, van der Krol AR, Oksman-Caldentey KM, Ratcliffe RG, Sweetlove LJ.
Phytochemistry. 2014 Mar;99:73-85. doi: 10.1016/j.phytochem.2013.12.007. Epub 2014 Jan 25.
http://www.ncbi.nlm.nih.gov/pubmed/24472392


Reduction of subacute endotoxemia could mediate beneficial effects of Vaccinium myrtillus in metabolic syndrome - A PBMC gene expression study.
Mykkänen OT, Kirjavainen PV, Pulkkinen L, Mykkänen H, Törrönen R, Kolehmainen M, Adriaens M, Laaksonen DE, Puupponen-Pimiä R, Poutanen K.
Acta Horticulturae. (2014) vol. 1017, pp. 369 - 380. ISBN 978-946261002-6
http://www.actahort.org/members/showpdf?booknrarnr=1017_45


Scale-up of hydrophobin-assisted recombinant protein production in tobacco BY-2 suspension cells.
Reuter LJ, Bailey MJ, Joensuu JJ, Ritala A.
Plant Biotechnol J. 2014 May;12(4):402-10. doi: 10.1111/pbi.12147. Epub 2013 Dec 17.
http://www.ncbi.nlm.nih.gov/pubmed/24341724


Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody.
Häkkinen ST, Raven N, Henquet M, Laukkanen ML, Anderlei T, Pitkänen JP, Twyman RM, Bosch D, Oksman-Caldentey KM, Schillberg S, Ritala A.
Biotechnol Bioeng. 2014 Feb;111(2):336-46. doi: 10.1002/bit.25113. Epub 2013 Sep 24.
http://www.ncbi.nlm.nih.gov/pubmed/24030771


2013


Analysis of the interface between primary and secondary metabolism in Catharanthus roseus cell cultures using (13)C-stable isotope feeding and coupled mass spectrometry.
Antonio C, Mustafa NR, Osorio S, Tohge T, Giavalisco P, Willmitzer L, Rischer H, Oksman-Caldentey KM, Verpoorte R, Fernie AR.
Mol Plant. 2013 Mar;6(2):581-4. doi: 10.1093/mp/sss156. Epub 2012 Dec 19.
http://www.ncbi.nlm.nih.gov/pubmed/23253601


Wave-mixed and orbitally shaken single-use photobioreactors for diatom algae propagation.
Lehmann N, Rischer H, Eibl D, Eibl R.
Chemie Ingenieur Technik.(2013) 85: 1–6. DOI:10.1002/cite.201200137
http://onlinelibrary.wiley.com/doi/10.1002/cite.201200137/abstract


The effect of high pH on structural lipids in diatoms.
Spilling K, Brynjólfsdóttir Á, Enss D, Rischer H, Svavarsson HG.
J. Appl. Phycol. (2013) 25:1435–1439. DOI 10.1007/s10811-012-9971-5
http://link.springer.com/article/10.1007%2Fs10811-012-9971-5
 
Metabolic engineering of plant secondary products: which way forward?
Miralpeix B, Rischer H, Häkkinen ST, Ritala A, Seppänen-Laakso T, Oksman-Caldentey KM, Capell T, Christou P.
Curr Pharm Des. 2013;19(31):5622-39.
http://www.ncbi.nlm.nih.gov/pubmed/23394556


CathaCyc, a metabolic pathway database built from Catharanthus roseus RNA-Seq data.
Van Moerkercke A, Fabris M, Pollier J, Baart GJ, Rombauts S, Hasnain G, Rischer H, Memelink J, Oksman-Caldentey KM, Goossens A.
Plant Cell Physiol. 2013 May;54(5):673-85. doi: 10.1093/pcp/pct039. Epub 2013 Mar 14.
http://www.ncbi.nlm.nih.gov/pubmed/23493402


Effects of ellagitannin-rich berries on blood lipids, gut microbiota, and urolithin production in human subjects with symptoms of metabolic syndrome.
Puupponen-Pimiä R, Seppänen-Laakso T, Kankainen M, Maukonen J, Törrönen R, Kolehmainen M, Leppänen T, Moilanen E, Nohynek L, Aura AM, Poutanen K, Tómas-Barberán FA, Espín JC, Oksman-Caldentey KM.
Mol Nutr Food Res. 2013 Dec;57(12):2258-63. doi: 10.1002/mnfr.201300280. Epub 2013 Aug 12.
http://www.ncbi.nlm.nih.gov/pubmed/23934737


Plant cells as pharmaceutical factories.
Rischer H, Häkkinen ST, Ritala A, Seppänen-Laakso T, Miralpeix B, Capell T, Christou P, Oksman-Caldentey KM.
Curr Pharm Des. 2013;19(31):5640-60.
http://www.ncbi.nlm.nih.gov/pubmed/23394561


Lipid production in 19 brackish and marine microalgae: influence by growth phase, salinity and temperature.
Schwenk D, Seppälä J, Spilling K, Virkki A, Tamminen T, Oksman-Caldentey K-M, Rischer H.
Aquat. Ecol. (2013) 47: 415-424. DOI 10.1007/s10452-013-9454-z
http://link.springer.com/article/10.1007%2Fs10452-013-9454-z


Mixotrophic continuous flow cultivation of Chlorella protothecoides for lipids.
Wang Y, Rischer H, Eriksen NT, Wiebe MG.
Bioresour Technol. 2013 Sep;144:608-14. doi: 10.1016/j.biortech.2013.07.027. Epub 2013 Jul 12.
http://www.ncbi.nlm.nih.gov/pubmed/23907064


Medicinal Plants, engineering of secondary metabolites in cell cultures.
Häkkinen ST, Ritala A, Rischer H, Oksman-Caldentey K-M.
(2013) pp.1182-1200.
In: Encyclopedia of sustainability science and technology.
P. Christou & R. Savin (Section eds.)
Springer-Verlag, Berlin Heidelberg, DOI:10.1007/SpringerReference_30127, ISBN: 978-1-4614-5796-1.

2012


In-depth proteome mining of cultured Catharanthus roseus cells identifies candidate proteins involved in the synthesis and transport of secondary metabolites.
Champagne A, Rischer H, Oksman-Caldentey KM, Boutry M.
Proteomics. 2012 Dec;12(23-24):3536-47. doi: 10.1002/pmic.201200218. Epub 2012 Nov 8.
http://www.ncbi.nlm.nih.gov/pubmed/23044725


Differential patterns of dehydroabietic acid biotransformation by Nicotiana tabacum and Catharanthus roseus cells.
Häkkinen ST, Lackman P, Nygrén H, Oksman-Caldentey KM, Maaheimo H, Rischer H.
J Biotechnol. 2012 Jan 20;157(2):287-94. doi: 10.1016/j.jbiotec.2011.11.008. Epub 2011 Nov 22.
http://www.ncbi.nlm.nih.gov/pubmed/22178236


Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.).
Dehghan E, Häkkinen ST, Oksman-Caldentey K-M, Shariari F.
Plant Cell Tissue Org. Cult. (2012) 110: 35-44. DOI 10.1007/s11240-012-0127-8.
http://link.springer.com/article/10.1007%2Fs11240-012-0127-8


Bilberries reduce low-grade inflammation in individuals with features of metabolic syndrome.
Kolehmainen M, Mykkänen O, Kirjavainen PV, Leppänen T, Moilanen E, Adriaens M, Laaksonen DE, Hallikainen M, Puupponen-Pimiä R, Pulkkinen L, Mykkänen H, Gylling H, Poutanen K, Törrönen R.
Mol Nutr Food Res. 2012 Oct;56(10):1501-10. doi: 10.1002/mnfr.201200195. Epub 2012 Sep 7.
http://www.ncbi.nlm.nih.gov/pubmed/22961907
 

2011


Drug metabolome of the simvastatin formed by human intestinal microbiota in vitro.
Aura AM, Mattila I, Hyötyläinen T, Gopalacharyulu P, Bounsaythip C, Orešič M, Oksman-Caldentey KM.
Mol Biosyst. 2011 Feb;7(2):437-46. doi: 10.1039/c0mb00023j. Epub 2010 Nov 8.
http://www.ncbi.nlm.nih.gov/pubmed/21060933


Jasmonate signaling involves the abscisic acid receptor PYL4 to regulate metabolic reprogramming in Arabidopsis and tobacco.
Lackman P, González-Guzmán M, Tilleman S, Carqueijeiro I, Pérez AC, Moses T, Seo M, Kanno Y, Häkkinen ST, Van Montagu MC, Thevelein JM, Maaheimo H, Oksman-Caldentey KM, Rodriguez PL, Rischer H, Goossens A.
Proc Natl Acad Sci U S A. 2011 Apr 5;108(14):5891-6. doi: 10.1073/pnas.1103010108. Epub 2011 Mar 21.
http://www.ncbi.nlm.nih.gov/pubmed/21436041


Lingonberry (Vaccinium vitis-idaea) and European cranberry (Vaccinium microcarpon) proanthocyanidins: isolation, identification, and bioactivities.
Kylli P, Nohynek L, Puupponen-Pimiä R, Westerlund-Wikström B, Leppänen T, Welling J, Moilanen E, Heinonen M.
J Agric Food Chem. 2011 Apr 13;59(7):3373-84. doi: 10.1021/jf104621e. Epub 2011 Mar 3.
http://www.ncbi.nlm.nih.gov/pubmed/21370878


Engineering of secondary metabolites in cell cultures of medicinal plants.
Häkkinen ST, Ritala A, Rischer H, Oksman-Caldentey K-M.
In: Encyclopedia of sustainability science and technology – section crop science and technology, (2011)
Christou P, Savin R (eds.), Springer Science + Business Media, LLC.

2010


Evidence for species differentiation within the Ancistrocladus tectorius complex (Ancistrocladaceae) in Southeast Asia: A molecular approach.
Meimberg M, Rischer H, Turini FG, Chamchumroon V, Dreyer M, Sommaro M, Bringmann G, Heubl G.
Plant Syst. Evol. (2010) 284: 77-98. DOI 10.1007/s00606-009-0241-1
http://link.springer.com/article/10.1007%2Fs00606-009-0241-1


Propagation of Triphyophyllum peltatum (Dioncophyllaceae) and observations on its carnivory.
Rembold K, Irmer A, Poppinga S, Rischer H, Bringmann G.
Carnivorous Plant Newsletter (2010) 39: 71-77.

Rowanberry phenolics: compositional analysis and bioactivities.
Kylli P, Nohynek L, Puupponen-Pimiä R, Westerlund-Wikström B, McDougall G, Stewart D, Heinonen M.
J Agric Food Chem. 2010 Nov 24;58(22):11985-92. doi: 10.1021/jf102739v. Epub 2010 Nov 1.
http://www.ncbi.nlm.nih.gov/pubmed/21038891
 
Culture establishment, plant cell.
Eibl R, Eibl D, Rischer H, Ritala A, Oksman-Caldentey K-M
(2010) pp. 1-11.
In: Encyclopedia of industrial biotechnology: Bioprocess, bioseparation and cell technology.
M.C. Flickinger (ed.)
John Wiley & Sons, Hoboken, New Jersey, DOI 10.1002/9780470054581.eib 247 (ISBN 978-0-471-79930-6).

Medicinal compounds produced in plant cell factories.
Häkkinen ST, Ritala A.
(2010) pp. 13-35.
In: Medicinal Plant Biotechnology.
Arora R (ed.)
CAB International, Oxfordshire, United Kingdom.

 

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