Anna-Marja Aura, D.Sc.
VTT Technical Research
Centre of Finland
Tietotie 2, P.O.Box 1000
FI-02044 VTT, Espoo
Phone: + 358 20 722 6178, Fax: +358 20 722 7071
The in vitro digestion models have been applied to microbial metabolism of
phytosterols, dietary fibre, lignans, isoflavonoids, flavonoids (catechins,
anthocyanins, flavonols), phenolic acids and tannins (ellagitannins and
condensed tannins). The analytical techniques used in the studies include
targeted analyses using LC-MS or GC-MS, and a non-targeted metabolomics
approach using GCxGC-TOF. European and national projects have involved
connection of dietary components with colon cancer, hormonal cancers,
cardiovascular diseases and diabetes (glycemia, insulinemia). The team
Health-benefiting Microbes has been involved in lactic acid bacterial and
human gut microbiota research for 10 years. During this time it has
participated and co-ordinated both internationally and nationally funded
projects. In gut microbiota studies both anaerobic culture-based (e.g. when
the survival of a specific bacterial strain through GI-tract is studied) and
molecular techniques from the population to the strains level have been used.
Of special interest are clostridia and related bacteria as well as
health-promoting bifidobacteria and lactobacilli.
Key people in the team
Dr. Anna-Marja Aura
Dr. Maria Saarela
Aura AM, Karppinen S, Virtanen H, Forssell P, Heinonen S-M, Nurmi T,
Adlercreutz H, Poutanen K. Processing of rye bran influences both the
fermentation of dietary fibre and the bioconversion of lignans by human faecal
flora in vitro. J Sci Food Agric 85, 2085-2093 (2005).
Aura AM, Oikarinen S, Mutanen M, Heinonen S-M, Adlercreutz HCT, Virtanen H,
Poutanen KS. Suitability of an in vitro fermentation model using human faecal
microbiota in prediction of conversion of flaxseed lignans to enterolactone in
reference to an in vivo rat model. Eur J Nutr 45, 45-51 (2006).
Aura AM, Mattila I, Seppänen-Laakso T, Miettinen J, Oksman-Caldentey K-M,
Oresic M, Microbial metabolism of catechin stereoisomers by human faecal
microbiota: comparison of targeted analysis and a non-targeted metabolomics
method. Phytochem Lett. In press.
Maukonen J, Mättö J, Satokari R, Söderlund H, Mattila-Sandholm T, Saarela M,
PCR-DGGE and RT-PCR-DGGE show diversity and short-term temporal stability in
the Clostridium coccoides - Eubacterium rectale group in the human intestinal
microbiota. FEMS Microbiol Ecol 58, 517-528 (2006).
Maukonen J, Satokari R, Mättö J, Söderlund H, Mattila-Sandholm T, Saarela M,
Prevalence and temporal stability of selected clostridial groups in irritable
bowel syndrome in relation to predominant fecal bacteria. J Med Microbiol 55,