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VTT Enzymes

VTT develops enzymes for industrial biotechnology applications and carries out research on various aspects of applied enzymology. This includes discovery of novel activities, characterization of their reaction mechanisms as well as improvement of enzyme properties through modern protein engineering techniques.

We discover novel enzymes for diverse applications depending on client’s needs. We have long and profound expertise especially on lignocellulose hydrolyzing enzymes and oxidative enzymes including laccases, tyrosinases, sulfhydryloxidases and lipoxygenases. We have also worked on other hydrolases, such as α-amylases, chitinases, esterases and lipases.

Classical screening methods, as well as modern techniques based on genomes and metagenomics can be used. High-throughput screening methods of the enzymes can be developed by using our robotic system. The production of enzymes can be done in various hosts in shake flask or in fermentors up to a volume of 1000 L. VTT is well-equipped for state of the art enzyme research, having e.g. ÄKTA protein purification units up to the pilot scale, and HPLC, MALDI-ToF MS, Surface Plasmon Resonance (SPR), fluorescence and CD spectroscopy facilities for enzyme characterisation.

Patents

  • WO12136898 Novel cutinases, their production and uses.
  • WO09007510 Novel esterases and their use.
  • WO06084953 Novel microbial enzymes and their use.
  • WO0192498 Novel laccase enzyme and the gene encoding the enzyme.

Selected publications

    Enzyme screening, engineering and discovery

  • Nakamura, A., Tsukada, T., Auer,S., Furuta, T., Wada, M., Koivula,A., Igarashi, K., and Samejima M. (2013) Tryptophan residue at active-site tunnel entrance of Trichoderma reesei cellobiohydrolase Cel7A is important to initiate degradation of crystalline cellulose.- In press, J.Biol.Chem. doi: 10.1074/jbc.M113.452623.
  • Nyyssölä A, Pihlajaniemi V, Järvinen R, Mikander S, Kontkanen H, Kruus K, Kallio H, Buchert J. Screening of microbes for novel acidic cutinases and cloning and expression of an acidic cutinase from Aspergillus niger CBS 513.88. Enz Microbial Technol (2013) http://dx.doi.org/10.1016/j.enzmictec.2013.01.005
  • Sanni P. Voutilainen, Harry Boer, Marika Alapuranen, Janne Jänis, Jari Vehmaanperä and Anu Koivula, Improving the thermostability and activity of Melanocarpus albomyces cellobiohydrolase Cel7B. Applied Microbiology and Biotechnology (2009), 83:261–272
  • Andberg M., Maaheimo H., Boer H., Penttilä M., Koivula A., and Richard P. Characterisation of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate. J Biol Chem. (2012), 287, 17662-17671
  • Harry Boer, Hannu Maaheimo, Anu Koivula, Merja Penttilä, and Peter Richard, Identification in Agrobacterium tumefaciens of the D-galacturonic acid dehydrogenase gene. Applied Microbiology and Biotechnology (2010), 86:901-9
  • Cellulase/hemicellulose/lignin

  • Viikari, L., Vehmaanperä, J. and Koivula, A (2012) Lignocellulosic ethanol: from science to industry. – Biomass and Bioenergy, in press.
  • Igarashi, K, Uchihashi, T, Koivula, A, Wada, M, Kimura, S, Okamoto, T, Penttilä, M, Ando, T, Samejima, M. Traffic jams reduce hydrolytic efficiency of cellulase on cellulose surface. Science. (2011) 333, 1279-1282.
  • Marjamaa K, Toth K, Bromann PA, Szakacs G, Kruus K (2013) Novel Penicillium cellulases for total hydrolysis of lignocellulosics. Enzyme and Microbial Technology, in press.
  • Sanni Voutilainen, Harry Boer, Markus Linder, Terhi Puranen, Juha Rouvinen, Jari Vehmaanperä and Anu Koivula, Heterologous expression of Melanocarpus albomyces cellobiohydrolase Cel7B, and random mutagenesis to improve its thermostability. Enzyme and Microbial Technology (2007) 41, 234-243
  • Rahikainen, J.L., Martin-Sampedro, R., Heikkinen, H., Rovio, S., Marjamaa, K., Tamminen, T., Rojas, O.J., Kruus, K. 2013. Inhibitory effect of lignin during cellulose bioconversion: the effect of lignin chemistry on non-productive enzyme adsorption. Bioresource Technology DOI: 10.1016/j.biortech.2013.01.075
  • Biosensor/fuel cell applications of enzyme

  • Maria Smolander, Harry Boer, Matti Valkiainen, Robert Roozeman, Mikael Bergelin, Jan-Erik Eriksson, Xia-Chang Zhang, Anu Koivula and Liisa Viikari, Development of a printable laccase based biocathode for fuel cell applications. Enzyme and Microbial Technology (2008), 43, 93-102
  • Sosna, M., Boer, H. and Bartlett, P. N. (2013), A His-Tagged Melanocarpus albomyces Laccase and its Electrochemistry upon Immobilisation on NTA-Modified Electrodes and in Conducting Polymer Films. ChemPhysChem, 14: 2225–2231
  • Xiaoju Wang, Rose-Marie Latonen, Pia Sjöberg-Eerola, Jan-Erik Eriksson, Johan Bobacka, Harry Boer, Mikael Bergelin, Direct Electron Transfer of Trametes Hirsuta Laccase in a Dual-Layer-Architecture of Poly(3,4-Ethylenedioxythiophene) Films. J. Phys. Chem. (2011) 115, 5919–5929


Additional information

Timo Pulli
Research Team Leader - Protein Discovery and Engineering
+358 40 720 7152

Additional information

Timo Pulli
Research Team Leader - Protein Discovery and Engineering
+358 40 720 7152